protein free blocking solution Search Results


proteinree blocking buffer  (LI-COR)
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LI-COR proteinree blocking buffer
Proteinree Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunohistochemistry protein blocker serum  (Rockland Immunochemicals)
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Rockland Immunochemicals immunohistochemistry protein blocker serum
Expression of B23 in bladder urothelial carcinoma and adjacent non-cancerous tissues, and normal human bladder and bladder cancer cells. (A) B23 expression in carcinoma and adjacent non-cancerous tissues was detected by immunohistochemical staining. (B) The percentage of B23-positive cases in 95 tumor tissues and the matched adjacent tissues was detected by <t>immunohistochemistry</t> analysis. There was a statistically significant increase in B23 staining between bladder urothelial carcinoma tissues and adjacent tissues. **P<0.01. (C) Protein and (D) mRNA expression levels of B23 in normal bladder tissues and tumors from bladder urothelial carcinoma patients were detected via western blotting and RT-qPCR, respectively. Protein and mRNA expression levels of B23 were increased in carcinoma tissues compared with adjacent tissues. (E) Protein and (F) mRNA expression levels of B23 in SV-HUC-1 normal human bladder cells and RT-4, BI-87, 253 J, J82 and T24 bladder cancer cell lines were detected by western blotting and RT-qPCR, respectively. B23 was highly expressed in bladder cancer cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N, normal bladder tissues; T, tumor tissues.
Immunohistochemistry Protein Blocker Serum, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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intercept protein free blocking buffer  (LI-COR)
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Expression of B23 in bladder urothelial carcinoma and adjacent non-cancerous tissues, and normal human bladder and bladder cancer cells. (A) B23 expression in carcinoma and adjacent non-cancerous tissues was detected by immunohistochemical staining. (B) The percentage of B23-positive cases in 95 tumor tissues and the matched adjacent tissues was detected by <t>immunohistochemistry</t> analysis. There was a statistically significant increase in B23 staining between bladder urothelial carcinoma tissues and adjacent tissues. **P<0.01. (C) Protein and (D) mRNA expression levels of B23 in normal bladder tissues and tumors from bladder urothelial carcinoma patients were detected via western blotting and RT-qPCR, respectively. Protein and mRNA expression levels of B23 were increased in carcinoma tissues compared with adjacent tissues. (E) Protein and (F) mRNA expression levels of B23 in SV-HUC-1 normal human bladder cells and RT-4, BI-87, 253 J, J82 and T24 bladder cancer cell lines were detected by western blotting and RT-qPCR, respectively. B23 was highly expressed in bladder cancer cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N, normal bladder tissues; T, tumor tissues.
Intercept Protein Free Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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azure protein  (Azure Biosystems)
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Azure Biosystems azure protein
Expression of B23 in bladder urothelial carcinoma and adjacent non-cancerous tissues, and normal human bladder and bladder cancer cells. (A) B23 expression in carcinoma and adjacent non-cancerous tissues was detected by immunohistochemical staining. (B) The percentage of B23-positive cases in 95 tumor tissues and the matched adjacent tissues was detected by <t>immunohistochemistry</t> analysis. There was a statistically significant increase in B23 staining between bladder urothelial carcinoma tissues and adjacent tissues. **P<0.01. (C) Protein and (D) mRNA expression levels of B23 in normal bladder tissues and tumors from bladder urothelial carcinoma patients were detected via western blotting and RT-qPCR, respectively. Protein and mRNA expression levels of B23 were increased in carcinoma tissues compared with adjacent tissues. (E) Protein and (F) mRNA expression levels of B23 in SV-HUC-1 normal human bladder cells and RT-4, BI-87, 253 J, J82 and T24 bladder cancer cell lines were detected by western blotting and RT-qPCR, respectively. B23 was highly expressed in bladder cancer cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N, normal bladder tissues; T, tumor tissues.
Azure Protein, supplied by Azure Biosystems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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intercept pbs protein free blocking buffer li cor  (LI-COR)
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Expression of B23 in bladder urothelial carcinoma and adjacent non-cancerous tissues, and normal human bladder and bladder cancer cells. (A) B23 expression in carcinoma and adjacent non-cancerous tissues was detected by immunohistochemical staining. (B) The percentage of B23-positive cases in 95 tumor tissues and the matched adjacent tissues was detected by <t>immunohistochemistry</t> analysis. There was a statistically significant increase in B23 staining between bladder urothelial carcinoma tissues and adjacent tissues. **P<0.01. (C) Protein and (D) mRNA expression levels of B23 in normal bladder tissues and tumors from bladder urothelial carcinoma patients were detected via western blotting and RT-qPCR, respectively. Protein and mRNA expression levels of B23 were increased in carcinoma tissues compared with adjacent tissues. (E) Protein and (F) mRNA expression levels of B23 in SV-HUC-1 normal human bladder cells and RT-4, BI-87, 253 J, J82 and T24 bladder cancer cell lines were detected by western blotting and RT-qPCR, respectively. B23 was highly expressed in bladder cancer cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N, normal bladder tissues; T, tumor tissues.
Intercept Pbs Protein Free Blocking Buffer Li Cor, supplied by LI-COR, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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buffer pbs  (LI-COR)
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LI-COR buffer pbs
Expression of B23 in bladder urothelial carcinoma and adjacent non-cancerous tissues, and normal human bladder and bladder cancer cells. (A) B23 expression in carcinoma and adjacent non-cancerous tissues was detected by immunohistochemical staining. (B) The percentage of B23-positive cases in 95 tumor tissues and the matched adjacent tissues was detected by <t>immunohistochemistry</t> analysis. There was a statistically significant increase in B23 staining between bladder urothelial carcinoma tissues and adjacent tissues. **P<0.01. (C) Protein and (D) mRNA expression levels of B23 in normal bladder tissues and tumors from bladder urothelial carcinoma patients were detected via western blotting and RT-qPCR, respectively. Protein and mRNA expression levels of B23 were increased in carcinoma tissues compared with adjacent tissues. (E) Protein and (F) mRNA expression levels of B23 in SV-HUC-1 normal human bladder cells and RT-4, BI-87, 253 J, J82 and T24 bladder cancer cell lines were detected by western blotting and RT-qPCR, respectively. B23 was highly expressed in bladder cancer cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N, normal bladder tissues; T, tumor tissues.
Buffer Pbs, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tbs t  (LI-COR)
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LI-COR tbs t
Expression of B23 in bladder urothelial carcinoma and adjacent non-cancerous tissues, and normal human bladder and bladder cancer cells. (A) B23 expression in carcinoma and adjacent non-cancerous tissues was detected by immunohistochemical staining. (B) The percentage of B23-positive cases in 95 tumor tissues and the matched adjacent tissues was detected by <t>immunohistochemistry</t> analysis. There was a statistically significant increase in B23 staining between bladder urothelial carcinoma tissues and adjacent tissues. **P<0.01. (C) Protein and (D) mRNA expression levels of B23 in normal bladder tissues and tumors from bladder urothelial carcinoma patients were detected via western blotting and RT-qPCR, respectively. Protein and mRNA expression levels of B23 were increased in carcinoma tissues compared with adjacent tissues. (E) Protein and (F) mRNA expression levels of B23 in SV-HUC-1 normal human bladder cells and RT-4, BI-87, 253 J, J82 and T24 bladder cancer cell lines were detected by western blotting and RT-qPCR, respectively. B23 was highly expressed in bladder cancer cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N, normal bladder tissues; T, tumor tissues.
Tbs T, supplied by LI-COR, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein-blocking solution  (BioShop)
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BioShop protein-blocking solution
Expression of B23 in bladder urothelial carcinoma and adjacent non-cancerous tissues, and normal human bladder and bladder cancer cells. (A) B23 expression in carcinoma and adjacent non-cancerous tissues was detected by immunohistochemical staining. (B) The percentage of B23-positive cases in 95 tumor tissues and the matched adjacent tissues was detected by <t>immunohistochemistry</t> analysis. There was a statistically significant increase in B23 staining between bladder urothelial carcinoma tissues and adjacent tissues. **P<0.01. (C) Protein and (D) mRNA expression levels of B23 in normal bladder tissues and tumors from bladder urothelial carcinoma patients were detected via western blotting and RT-qPCR, respectively. Protein and mRNA expression levels of B23 were increased in carcinoma tissues compared with adjacent tissues. (E) Protein and (F) mRNA expression levels of B23 in SV-HUC-1 normal human bladder cells and RT-4, BI-87, 253 J, J82 and T24 bladder cancer cell lines were detected by western blotting and RT-qPCR, respectively. B23 was highly expressed in bladder cancer cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N, normal bladder tissues; T, tumor tissues.
Protein Blocking Solution, supplied by BioShop, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rapid blocking solution  (Beijing Solarbio Science)
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Beijing Solarbio Science rapid blocking solution
Expression of B23 in bladder urothelial carcinoma and adjacent non-cancerous tissues, and normal human bladder and bladder cancer cells. (A) B23 expression in carcinoma and adjacent non-cancerous tissues was detected by immunohistochemical staining. (B) The percentage of B23-positive cases in 95 tumor tissues and the matched adjacent tissues was detected by <t>immunohistochemistry</t> analysis. There was a statistically significant increase in B23 staining between bladder urothelial carcinoma tissues and adjacent tissues. **P<0.01. (C) Protein and (D) mRNA expression levels of B23 in normal bladder tissues and tumors from bladder urothelial carcinoma patients were detected via western blotting and RT-qPCR, respectively. Protein and mRNA expression levels of B23 were increased in carcinoma tissues compared with adjacent tissues. (E) Protein and (F) mRNA expression levels of B23 in SV-HUC-1 normal human bladder cells and RT-4, BI-87, 253 J, J82 and T24 bladder cancer cell lines were detected by western blotting and RT-qPCR, respectively. B23 was highly expressed in bladder cancer cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N, normal bladder tissues; T, tumor tissues.
Rapid Blocking Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein free blocking buffer  (GenTel BioSurfaces)
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GenTel BioSurfaces protein free blocking buffer
Expression of B23 in bladder urothelial carcinoma and adjacent non-cancerous tissues, and normal human bladder and bladder cancer cells. (A) B23 expression in carcinoma and adjacent non-cancerous tissues was detected by immunohistochemical staining. (B) The percentage of B23-positive cases in 95 tumor tissues and the matched adjacent tissues was detected by <t>immunohistochemistry</t> analysis. There was a statistically significant increase in B23 staining between bladder urothelial carcinoma tissues and adjacent tissues. **P<0.01. (C) Protein and (D) mRNA expression levels of B23 in normal bladder tissues and tumors from bladder urothelial carcinoma patients were detected via western blotting and RT-qPCR, respectively. Protein and mRNA expression levels of B23 were increased in carcinoma tissues compared with adjacent tissues. (E) Protein and (F) mRNA expression levels of B23 in SV-HUC-1 normal human bladder cells and RT-4, BI-87, 253 J, J82 and T24 bladder cancer cell lines were detected by western blotting and RT-qPCR, respectively. B23 was highly expressed in bladder cancer cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N, normal bladder tissues; T, tumor tissues.
Protein Free Blocking Buffer, supplied by GenTel BioSurfaces, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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serum-free protein blocking buffer  (ZSGB Biotech)
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Expression of B23 in bladder urothelial carcinoma and adjacent non-cancerous tissues, and normal human bladder and bladder cancer cells. (A) B23 expression in carcinoma and adjacent non-cancerous tissues was detected by immunohistochemical staining. (B) The percentage of B23-positive cases in 95 tumor tissues and the matched adjacent tissues was detected by <t>immunohistochemistry</t> analysis. There was a statistically significant increase in B23 staining between bladder urothelial carcinoma tissues and adjacent tissues. **P<0.01. (C) Protein and (D) mRNA expression levels of B23 in normal bladder tissues and tumors from bladder urothelial carcinoma patients were detected via western blotting and RT-qPCR, respectively. Protein and mRNA expression levels of B23 were increased in carcinoma tissues compared with adjacent tissues. (E) Protein and (F) mRNA expression levels of B23 in SV-HUC-1 normal human bladder cells and RT-4, BI-87, 253 J, J82 and T24 bladder cancer cell lines were detected by western blotting and RT-qPCR, respectively. B23 was highly expressed in bladder cancer cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N, normal bladder tissues; T, tumor tissues.
Serum Free Protein Blocking Buffer, supplied by ZSGB Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Expression of B23 in bladder urothelial carcinoma and adjacent non-cancerous tissues, and normal human bladder and bladder cancer cells. (A) B23 expression in carcinoma and adjacent non-cancerous tissues was detected by immunohistochemical staining. (B) The percentage of B23-positive cases in 95 tumor tissues and the matched adjacent tissues was detected by immunohistochemistry analysis. There was a statistically significant increase in B23 staining between bladder urothelial carcinoma tissues and adjacent tissues. **P<0.01. (C) Protein and (D) mRNA expression levels of B23 in normal bladder tissues and tumors from bladder urothelial carcinoma patients were detected via western blotting and RT-qPCR, respectively. Protein and mRNA expression levels of B23 were increased in carcinoma tissues compared with adjacent tissues. (E) Protein and (F) mRNA expression levels of B23 in SV-HUC-1 normal human bladder cells and RT-4, BI-87, 253 J, J82 and T24 bladder cancer cell lines were detected by western blotting and RT-qPCR, respectively. B23 was highly expressed in bladder cancer cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N, normal bladder tissues; T, tumor tissues.

Journal: Molecular Medicine Reports

Article Title: High expression of B23 is associated with tumorigenesis and poor prognosis in bladder urothelial carcinoma

doi: 10.3892/mmr.2016.6033

Figure Lengend Snippet: Expression of B23 in bladder urothelial carcinoma and adjacent non-cancerous tissues, and normal human bladder and bladder cancer cells. (A) B23 expression in carcinoma and adjacent non-cancerous tissues was detected by immunohistochemical staining. (B) The percentage of B23-positive cases in 95 tumor tissues and the matched adjacent tissues was detected by immunohistochemistry analysis. There was a statistically significant increase in B23 staining between bladder urothelial carcinoma tissues and adjacent tissues. **P<0.01. (C) Protein and (D) mRNA expression levels of B23 in normal bladder tissues and tumors from bladder urothelial carcinoma patients were detected via western blotting and RT-qPCR, respectively. Protein and mRNA expression levels of B23 were increased in carcinoma tissues compared with adjacent tissues. (E) Protein and (F) mRNA expression levels of B23 in SV-HUC-1 normal human bladder cells and RT-4, BI-87, 253 J, J82 and T24 bladder cancer cell lines were detected by western blotting and RT-qPCR, respectively. B23 was highly expressed in bladder cancer cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N, normal bladder tissues; T, tumor tissues.

Article Snippet: Non-specific antigen-antibody reactions were inhibited using an immunohistochemistry Protein Blocker-serum and Azide Free (MB-071-0100, Rockland Immunochemicals, Inc., Pottstown, PA, USA) for 5 min, following which the slides were washed thoroughly with PBS.

Techniques: Expressing, Immunohistochemical staining, Staining, Immunohistochemistry, Western Blot, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction